anti-CD71 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD71; anti-CD71 antibody;
The CD71 n/a (Catalog #MBS666616) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD71-Biotin reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD71 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), (Not WB). Researchers should empirically determine the suitability of the CD71 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD71-Biotin with the following immunoassay(s):
Testing Data
Information: Human CD71 is a type II transmembrane glycoprotein found on activated leukocytes. CD71 binds transferrin and mediates uptake of iron through endocytosis and recycling. Antibody DF1513 recognizes the CD71 molecule of about 95 kd.
Performance: Five x 105 cultured HPB-MLT cells were washed and incubated 45 minutes on ice with 80 mul of anti-CD71/Biotin at 5 mug/ml. Cells were washed twice and incubated with Streptavidin/R-Phycoerythrin, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.9 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated with 20mul of 0.5mg/ml unlabeled anti-CD71 mAb. Conjugation: Biotin
Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen: Human PBL. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD71 are readily searchable from our website. Different antibodies against the same target such as CD71 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.