Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Monoclonal Antibody Blog  rightarrow anti-CD73 antibody  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • anti-CkIIbeta antibody blog
 • anti-HSP90AA1 antibody blog
 • anti-Nod2 antibody blog
 • anti-IL-28A antibody blog
 • anti-AMH antibody blog
 • anti-BMPR2 antibody blog
 • anti-Influenza B Virus antibody blog
 • anti-Salbutamol antibody blog
 • anti-PSMB7 antibody blog
 • anti-Pan-Kvbeta1.1 K+ Channel antibody blog
 • anti-CD357 antibody blog
 • anti-USP7 antibody blog
 • anti-MYCOPLASMA PNEUMONIAE P1 antibody blog
 • anti-TNFa antibody blog
 • anti-FKRP antibody blog
 • anti-KRT5 antibody blog
 • anti-MKK3/6 antibody blog
 • anti-TRAP1 antibody blog
 • anti-c-Kit antibody blog
 • anti-CD105 antibody blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • CD64 Antibody
 • HLA Class 1 Antigen B13,B62,B15 Antibody
 • Soluble Cluster of Differentiation 86 (SCD86) ELISA Kit
 • KLK7 Antibody
 • Hemoglobin (RB1909) Antibody
 • Complement regulatory protein Crry (Crry) Recombinant Protein
 • dorsal root ganglia homeobox (DRGX) ELISA Kit
 • treponema pallidun (TP) antibody (IgM) ELISA Kit
 • 6-phosphofructo-2-kinase-fr uctose-2,6-bisphosphatase 4 (PFKF... Recombinant Protein
 • Prostate Specific Antigen, Free Antibody
 • 1-acyl-sn-glycerol-3-phosph ate acyltransferase alpha (AGPAT1... ELISA Kit
 • Myb (Phospho-Ser532) Antibody
 • SGSH Antibody
 • Lactoferrin (LTF) Antibody
 downarrow more ...
separator

anti-CD73 antibody product blog

separator
Posted on 2015-10-21 22:34:30 by mybiosource_staff
Share it on ...     
separator
separator
Tags: Antibody; Monoclonal Antibody; CD73; anti-CD73 antibody;
separator
The CD73 n/a (Catalog #MBS584066) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD73, Human, mAb 4G4 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD73 can be used in a range of immunoassay formats including, but not limited to, F, Flow Cytometry (FC/FACS), FS, Immunoflurescence (IF), Immunoprecipitation (IP), Western Blot (WB).
Application Notes: F:- Tissue sections were fixed in acetone.- As positive control anti-CD31 was used and as negative control an isotype antibody (Ref.5).
Application Use: For immunohistology, and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user\'s experimental setting. Researchers should empirically determine the suitability of the CD73 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Product Description: The monoclonal antibody 4G4 recognizes both membrane bound and soluble human CD73, also known as ecto-5-nucleotidase. CD73 is a 70-kDa GPI-anchored cell surface molecule and belongs to the 5-nucleosidase family. CD73 is useful as marker for lymphocyte differentiation. It is abundantly expressed on the vascular endothelium and at a low level on certain subpopulations of human lymphocytes. Like many glycosyl-phosphatidylinositol (GPI)-anchored molecules, it transmits potent activation signals in T cells when ligated by antibodies. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Ecto-5-nucleotidase activity is an important mediator of the antiinflammatory effect by converting extracellular AMP into a potent anti-inflammatory substance adenosine. CD73 has been shown to function as a co-stimulatory molecule in human T cells and to have a role in regulating lymphocyte adhesion. Triggering of CD73 on the surface of lymphocytes, but not on endothelial cells, results in the shedding of the CD73 and increased adhesion of lymphocytes to endothelium via LFA-1 clustering. Furthermore, CD73 has been implicated to mediate homing of skin-infiltrating lymphocytes in vivo.
In B-cell chronic lymphocytic leukemia the expression of CD73 is decreased. Besides this, CD73 activity has been implicated as sensitive and useful indicator for mild zinc deficiency. The monoclonal antibody 4G4 causes a reduction in CD73 expression on lymphocytes, reduces enzyme activity, and inhibits the binding of lymphocytes to endothelial cells.

CD73 n/a

Immunogen: Inflamed synovial stroma from rheumatoid arthitis patients. Positive Control: HUVEC and fresh lymphocytes. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD73 are readily searchable from our website. Different antibodies against the same target such as CD73 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions