Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Monoclonal Antibody Blog  rightarrow anti-CD74 antibody  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • anti-TNFRII antibody blog
 • anti-PEG antibody blog
 • anti-Rb Protein antibody blog
 • anti-Prss22 antibody blog
 • anti-CD29 antibody blog
 • anti-Cacng2 antibody blog
 • anti-TFF2 antibody blog
 • anti-DECR1 antibody blog
 • anti-IghA1 antibody blog
 • anti-ALDH2 antibody blog
 • anti-RB1 antibody blog
 • anti-IL13 antibody blog
 • anti-Mycoplasma genitalium antibody blog
 • anti-CBX3 antibody blog
 • anti-LAMB1 antibody blog
 • anti-TNFRSF11B antibody blog
 • anti-G6PD antibody blog
 • anti-ERp57 antibody blog
 • anti-Gria2 antibody blog
 • anti-MHC antibody blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • L-lactate dehydrogenase A chain (LDHA) ELISA Kit
 • Fatty acid-binding protein (FABP) ELISA Kit
 • CytoSelect 48-well Cell Adhesion Assay (Laminin, Colorimetri... Assay Kit
 • Histone, H2A1, Macro (h2afy) Antibody
 • A Disintegrin And Metalloprotease 17 (ADAM17) Antibody
 • p16 INK ELISA Kit
 • 3-Hydroxy-3-methylglutaryl- Coenzyme A Synthase (HMGCS) ELISA Kit
 • Catenin alpha1 (CTNNA1) Antibody
 • Low affinity immunoglobulin gamma Fc region receptor II (Fcg... Recombinant Protein
 • Estriol ELISA Kit
 • Bovine IgG (Fc) Immunoglobulin
 • Herpes Simplex Virus-1 (HSV-1) Glycoprotein D Glycosylated, ... Recombinant Protein
 • Nascent polypeptide-associated complex subunit alpha, muscle... Recombinant Protein
 • ZGLP1 Peptide
 downarrow more ...
separator

anti-CD74 antibody product blog

separator
Posted on 2015-10-25 23:05:09 by mybiosource_staff
Share it on ...     
separator
separator
Tags: Antibody; Monoclonal Antibody; CD74; anti-CD74 antibody;
separator
The CD74 cd74 (Catalog #MBS212849) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD74 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen*, Flow cytometry (FC/FACS)*, Functional Assays (FN)*, Immunofluorescence (IF), Immunoprecipitation (IP).
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.
Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/50; Application Note: Membrane permeabilisation may enhance CD74 staining. MyBioSource recommend the use of LeucopermTM for this purpose.

Functional Assays: Application Note: "This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Dialysis cassettes. Researchers should empirically determine the suitability of the CD74 cd74 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD74 cd74 product has the following accession number(s) (GI #68448537) (NCBI Accession #NP_001020329.1) (Uniprot Accession #P04233). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Mouse anti Human CD74 antibody, clone Bu45 recognizes human CD74, a type II transmembrane protein, which is also known as the MHC class II associated invariant chain. The Bu45 antibody recognizes 33, 35 and 41 kD isoforms of CD74. The CD74 molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes. CD74 is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, dendritic cells and by various types of carcinoma. Expression of CD74 is primarily intracellular with moderate expression at the cell surface of B cells and monocytes. The Bu45 antibody binds to an epitope within the extracytoplasmic domain of the CD74 molecule, which is distal from the C-terminus.

CD74 cd74

Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Immunogen: B lymphoblastoid cell line HFB1.
Fusion Partners: Spleen cells from immunised mice were fused with cells of the Ag8.653 myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD74 are readily searchable from our website. Different antibodies against the same target such as CD74 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions