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anti-CD74 antibody product blog

Posted on 2015-10-25 23:05:09 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD74; anti-CD74 antibody;
The CD74 cd74 (Catalog #MBS212849) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD74 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen*, Flow cytometry (FC/FACS)*, Functional Assays (FN)*, Immunofluorescence (IF), Immunoprecipitation (IP).
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.
Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/50; Application Note: Membrane permeabilisation may enhance CD74 staining. MyBioSource recommend the use of LeucopermTM for this purpose.

Functional Assays: Application Note: "This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Dialysis cassettes. Researchers should empirically determine the suitability of the CD74 cd74 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD74 cd74 product has the following accession number(s) (GI #68448537) (NCBI Accession #NP_001020329.1) (Uniprot Accession #P04233). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Mouse anti Human CD74 antibody, clone Bu45 recognizes human CD74, a type II transmembrane protein, which is also known as the MHC class II associated invariant chain. The Bu45 antibody recognizes 33, 35 and 41 kD isoforms of CD74. The CD74 molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes. CD74 is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, dendritic cells and by various types of carcinoma. Expression of CD74 is primarily intracellular with moderate expression at the cell surface of B cells and monocytes. The Bu45 antibody binds to an epitope within the extracytoplasmic domain of the CD74 molecule, which is distal from the C-terminus.

CD74 cd74

Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Immunogen: B lymphoblastoid cell line HFB1.
Fusion Partners: Spleen cells from immunised mice were fused with cells of the Ag8.653 myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD74 are readily searchable from our website. Different antibodies against the same target such as CD74 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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