|The CD74 cd74 (Catalog #MBS9200181) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD74 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD74 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS).
WB~~1:1000. Researchers should empirically determine the suitability of the CD74 cd74 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD74 cd74 product has the following accession number(s) (GI #68448537) (NCBI Accession #NP_001020329.1) (Uniprot Accession #P04233). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CD74 Antibody with the following immunoassay(s):
Flow Cytometry (FC/FACS) (Flow cytometric analysis of Raji cells using CD74(green) compared to an isotype control of mouse IgG2b(blue). MBS9200181 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
Western Blot (WB) (Western blot analysis of lysates from Daudi, Raji cell line (from left to right), using CD74 Antibody. MBS9200181 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
Antigen Type: Recombinant Protein
Crown Antibody: Yes. Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD74 are readily searchable from our website. Different antibodies against the same target such as CD74 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Brain Diseases, Carcinoma, Carcinoma, Non-Small-Cell Lung, Chromosome Aberrations, Disease Models, Animal, Inflammation, Kidney Diseases, Lung Neoplasms, Necrosis, Neoplasms, Experimental are some of the diseases may be linked to CD74 Antibody.