|The CD8 cd8b (Catalog #MBS224781) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI PIG CD8 BETA CHAIN reacts with Pig and may cross-react with other species as described in the data sheet. MyBioSource\'s CD8 BETA CHAIN can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS).
Flow Cytometry: 1/50; 1/200. Researchers should empirically determine the suitability of the CD8 cd8b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD8 cd8b product has the following accession number(s) (GI #4261577) (NCBI Accession #AAD13877.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI PIG CD8 BETA CHAIN with the following immunoassay(s):
Flow Cytometry (FC/FACS) (Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain:RPE )
Flow Cytometry (FC/FACS) (Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain:FITC )
Flow Cytometry (FC/FACS) (Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD8 beta chain followed by Goat anti Mouse IgG (H/L):FITC )
Mouse anti Pig CD8 beta chain, clone PPT23, recognizes the porcine homologue of the human CD8 beta chain cell surface antigen.Characterization of clone PPT23, also known under the clone designation FYP1C5, has demonstrated that on peripheral blood lymphocytes and spleen, this antibody binds to cells that are CD3+, CD4-, CD8hi and as such defines this antibody as a specific marker of porcine alpha/beta T cells. Characterization of clone PPT23 has shown that in thymic tissue both CD8lo and CD8hi cells are recognized (Yang H & Parkhouse R.M. 1997). Inhibition studies have demonstrated that clone PPT23 recognizes a different epitope of on the CD8 beta chain to clone PPT22.
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preservative Stabilisers: 0.09% Sodium Azide
Buffer Solution: Phosphate buffered saline. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD8 are readily searchable from our website. Different antibodies against the same target such as CD8 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.