Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Monoclonal Antibody Blog  rightarrow anti-CD8 antibody  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • anti-Free PSA antibody blog
 • anti-MCP-3 antibody blog
 • anti-GS antibody blog
 • anti-Hspa5 antibody blog
 • anti-KCNQ4 antibody blog
 • anti-ACTB antibody blog
 • anti-Pax5 antibody blog
 • anti-MYOG antibody blog
 • anti-CD4 antibody blog
 • anti-HSPB2 antibody blog
 • anti-NFkB, p52 antibody blog
 • anti-GAD65 antibody blog
 • anti-CD86 antibody blog
 • anti-Cytokeratin 6 antibody blog
 • anti-MACROPHAGES/MONOCYTES antibody blog
 • anti-LAMP2 antibody blog
 • anti-Raf1 antibody blog
 • anti-CST3  antibody blog
 • anti-AMH antibody blog
 • anti-GLUL antibody blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Carcinoembryonic Antigen (CEA) Antibody
 • hypoxia inducible factor 1, alpha subunit (basic helix-loop-... ELISA Kit
 • p130 Antibody
 • PTD ELISA Kit
 • Glutathione Peroxidase 1 (GPX1) Peptide
 • Adenosine A2a Receptor (ADORA2a) ELISA Kit
 • A Disintegrin And Metalloprotease 12 (ADAM12) ELISA Kit
 • 5-Hydroxyindoleacetic Acid (5-HIAA) ELISA Kit
 • Bcl-xL Antibody
 • A disintegrin and metalloproteinase with thrombospondin moti... ELISA Kit
 • Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) Antibody
 • ASTROVIRUS (ORF2) Antibody
 • Angiostatin Kringles 1-3 Recombinant Protein
 • ketohexokinase (fructokinase) (KHK) Antibody
 downarrow more ...
separator

anti-CD8 antibody product blog

separator
Posted on 2018-06-07 12:48:36 by mybiosource_staff
Share it on ...     
separator
separator
Tags: Antibody; CD8; Monoclonal Antibody; anti-CD8 antibody;
separator
The CD8 n/a (Catalog #MBS8505597) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti-Human CD8, FITC Conjugated mAb reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD8 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the CD8 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD8 n/a product has the following accession number(s) (GI #76786711) (NCBI Accession #ABA55041.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


4ARiv11 reacts with the 32 kDa a chain of CD8. This molecule is expressed on the T suppressor/cytotoxic cell population (which comprises about 1/3 of the peripheral blood T lymphocytes total population) and with most of thymocytes, as well as a subset of NK cells. CD8 expresses as either a heterodimer with the CD8b chain (CD8ab) or as a homodimer (CD8aa or CD8bb). CD8 acts as a co-receptor with MHC Class I restricted TCRs in antigen recognition. CD8 function is important for positive selection of MHC Class I restricted CD8+ T cells during T cell development.* Ratio of CD4 and CD8 is important for monitor of HIV infection.

CD8 n/a

Experimental Methods: 1.Take 100mul peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add 10mul labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3]
[Cell fixation: 2% formaldehyde solution]. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD8 are readily searchable from our website. Different antibodies against the same target such as CD8 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions