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anti-CD80 antibody product blog

Posted on 2015-09-03 21:01:05 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD80; anti-CD80 antibody;
The CD80 n/a (Catalog #MBS666476) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD80 (B7-1)-R-PE reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD80 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA).
1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test). Researchers should empirically determine the suitability of the CD80 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD80 n/a product has the following accession number(s) (GI #119331110) (NCBI Accession #NP_001073207.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD80 (B7-1)-R-PE with the following immunoassay(s):
Testing Data
Testing Data CD80.

Information: Human CD80 (B7-1) is a costimulatory ligand for CD28 and CTLA-4. CD80 is expressed on activated B cells. Antibody P1.H5.A1.A1 recognizes the CD80 molecule and blocks binding of soluble CD152 Ig fusion protein to CD80.

Performance: Five x 105 cultured Raji cells were incubated 45 minutes on ice with 80 ul of anti-CD80/R-PE at a 1:50 dilution (20 ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.72 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD80 (P1.H5.A1.A1) antibody. Conjugation: PE
Production: Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 1.0 mg/ml with an A565/A280 ratio of 2.81
Buffer: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen: Human CD80 (B7-1) Ig Fusion Protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD80 are readily searchable from our website. Different antibodies against the same target such as CD80 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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