|The CD83 cd83 (Catalog #MBS609399) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD83 (PE) (B-cell activation protein, BL11, Cell Surface Protein HB15) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD83 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS).
Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: 1:50 (80ul/test)
Flow Cytometry Protocol: 1. Five x 10e5 human Raji cells per tube were washed and incubated for 45 minutes on ice with 80ul of C2435-04C at a dilution factor of 1:50.
2. Cells washed three times, fixed and analyzed using a BD FACS Calibur.
3. Cells stained positive with a mean shift of 0.6 log10 fluorescent units when compared to a mouse IgG1/R-PE negative control at a similar concentration.
4. Binding was 99% blocked when cells were pre-incubated for 10 minutes with 20ul of C2435-04 at 0.5mg/ml. Researchers should empirically determine the suitability of the CD83 cd83 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD83 cd83 product has the following accession number(s) (GI #4757946) (NCBI Accession #NP_004224.1) (Uniprot Accession #Q01151). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Human CD83 is a type 1 cell surface glycoprotein expressed almost solely by dendritic cells that play an important role in antigen presentation (2).
Immunogen: COS cells transfected with human CD83. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD83 are readily searchable from our website. Different antibodies against the same target such as CD83 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone Marrow, Kidney, Liver, Lung, Lymph, Lymph Node, Skin, Spleen, Umbilical Cord tissues are correlated with this protein. Adenocarcinoma, Hematologic Diseases, Hepatitis, Immune System Diseases, Inflammation, Liver Diseases, Neoplasms, Neuroectodermal Tumors, Skin Diseases, Vascular Diseases are some of the diseases may be linked to CD83 (PE) (B-cell activation protein, BL11, Cell Surface Protein HB15).