anti-CD86 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD86; anti-CD86 antibody;
The CD86 cd86 (Catalog #MBS210978) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD86 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS).Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Researchers should empirically determine the suitability of the CD86 cd86 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD86 cd86 product has the following accession number(s) (GI #161484599) (NCBI Accession #NP_062261.3) (Uniprot Accession #P42082). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RAT ANTI MOUSE CD86:FITC with the following immunoassay(s):
Testing Data (Staining of LPS stimulated mouse spleen cells with Rat anti Mouse CD86:FITC)
Rat anti mouse CD86 recognizes mouse CD86 (B7-2), an 80kD cell surface glycoprotein which is a member of the CD28/B7 family. In mouse CD86 is expressed at high levels on peripheral blood monocytes and dendritic cells, and at low levels on resting B and T-lymphocytes. Expression of CD86, on these cell populations, can be increased upon activation. CD86 has been identified, along with CD80 (B7-1), as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA4). CD28 and CTLA4 are two receptors that have opposing functions in T-cell stimulation. Interaction of CD86 with CD28 promotes a number of T-cell activities, whereas the binding of CD86 to CTLA4 inhibits T-cell responses. The PO3 antibody has been reported to block some co-stimulatory functions of CD86 �(Nakajima et al. 1997).
Perservative Stabilisers: 0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Immunogen: Mouse B-cell line, BCL1.
Fusion Partners: Spleen cells from immunised Sprague Dawley rats were fused with cells of the P3U1 myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Mouse. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD86 are readily searchable from our website. Different antibodies against the same target such as CD86 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.