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anti-CD86 antibody product blog

Posted on 2015-08-15 17:39:53 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD86; anti-CD86 antibody;
The CD86 cd86 (Catalog #MBS212031) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD86 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP).
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology - Frozen: Minimum Dilution: 1/20; Maximum Dilution: 1/100
Flow Cytometry: Minimum Dilution: 1/20; Maximum Dilution: 1/100. Researchers should empirically determine the suitability of the CD86 cd86 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD86 cd86 product has the following accession number(s) (GI #332634950) (NCBI Accession #NP_001193853.1) (Uniprot Accession #P42081). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI HUMAN CD86 with the following immunoassay(s):
Testing Data (Staining of human peripheral blood monocytes with Mouse anti Human CD86:FITC (MBS210618))
Testing Data CD86.

Testing Data (Staining of human peripheral blood monocytes with Mouse anti Human CD86:RPE (MBS213553))
Testing Data CD86.

Mouse anti Human CD86 antibody, clone Bu63 recognizes human CD86 also known as B7-2, a type I transmembrane protein expressed by monocytes and activated B cells (Engel et al. 1994). CD86 acts as a co-stimulaory molecule along with CD80 (Lanier et al. 1995) and is a ligand for CD28 and CTLA-4 (Azuma et al. 1993). CD86 is a member of the Immunoglobulin superfamily and carries an extracellular domain bearing both an Ig-v-like domain which contains the CTLA-4 binding site and an adjacent C2-like domain. CD86 plays an important role in co-stimulation of T cell proliferation (Freeman et al. 1993), IL-2 production (Ribot et al. 2012) and in the primary immune response (Schultze et al. 1996). Domain depletion epitope mapping studies indicate that the binding site of Mouse anti Human CD86, clone Bu63 is located within the Ig-v-like domain of human CD86 (Jeanin et al. 1997). Studies suggest that CD86 along with CD80 may be exploited as receptors for adenovirus entry into cells (Short et al. 2004 2006).

Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant. Histology Positive Control Tissue: Human Tonsil
Buffer Solution: Phosphate buffered saline
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD86 are readily searchable from our website. Different antibodies against the same target such as CD86 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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