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anti-CD86 antibody product blog

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Posted on 2015-07-25 17:22:57 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD86; anti-CD86 antibody;
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The CD86 cd86 (Catalog #MBS219503) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD86 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS).
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10. Researchers should empirically determine the suitability of the CD86 cd86 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD86 cd86 product has the following accession number(s) (GI #49456643) (NCBI Accession #CAG46642.1) (Uniprot Accession #P42081). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI RAT CD86:FITC with the following immunoassay(s):
Testing Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD86 antibody, clone 42F (MBS219747) followed by horseradish peroxidase conjugated Goat anti Mouse IgG (MBS235406). High power)
Testing Data CD86.

Testing Data (Staining of rat splenocytes with Mouse anti Rat CD86:Alexa Fluor ?488 (MBS219747A488))
Testing Data CD86.

Testing Data (Staining of rat splenocytes with Mouse anti Rat CD86:FITC)
Testing Data CD86.

Testing Data (Staining of rat splenocytes with Mouse anti Rat CD86:Low Endotoxin (MBS219602))
Testing Data CD86.

Testing Data (Staining of rat splenocytes with Mouse anti Rat CD86:Alexa Fluor ?647 (MBS219747A647))
Testing Data CD86.

Testing Data (Staining of rat splenocytes with Mouse anti Rat CD86:RPE (MBS219683))
Testing Data CD86.

Testing Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD86 antibody, clone 42F (MBS219747) followed by horseradish peroxidase conjugated Goat anti Mouse IgG (MBS235406). Medium power)
Testing Data CD86.

Testing Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD86 antibody, clone 42F (MBS219747) followed by horseradish peroxidase conjugated Goat anti Mouse IgG (MBS235406). Low power)
Testing Data CD86.

Mouse anti Rat CD86 antibody, clone 24F specifically recognizes rat CD86, otherwise known as B7-2, a type I transmembrane protein and member of the Ig superfamily, which acts as a ligand for both CD28 and CD152 (CTLA-4), and is primarily expressed on antigen presenting cells (APCs) including dendritic cells, and also on germinal centre B cells and macrophages. Like CD80, CD86 is an accessory molecule which functions in the CD28-CD80/CD86 co-stimulatory pathway, vital for T cell activation, crosstalk between T and B cells, and Th2-mediated Ig production. Mouse anti Rat CD86 antibody, clone 24F has been shown to block the co-stimulatory activity of rat CD86.

Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
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