anti-CD9 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD9; anti-CD9 antibody;
The CD9 cd9 (Catalog #MBS9602590) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD9 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA), Flow Cytometry (FC/FACS).ELISA: 1:10000
WB: 1:500-1:2000
IHC: 1:200-1:1000
FC/FACS: 1:200-1:400. Researchers should empirically determine the suitability of the CD9 cd9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD9 cd9 product has the following accession number(s) (GI #1057867401) (NCBI Accession #NP_001317241.1) (Uniprot Accession #P21926). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CD9 Antibody with the following immunoassay(s):
Western Blot (WB) (Figure 1: Western blot analysis using CD9 mAb against HEK293 (1) and CD9(AA: 37-228)-hIgGFc transfected HEK293 (2) cell lysate.)
Description: The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance.
Function: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
Subunit Structure: Forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family. Interacts with CD63. Identified in a complex with CD63 and ITGB3. Associates with CR2/CD21 and with PTGFRN/CD9P1. Interacts directly with IGSF8. Interacts with PDPN; this interaction is homophilic and attenuates platelet aggregation and pulmonary metastasis induced by PDPN (PubMed:18541721). Interacts (via the second extracellular domain) with integrin ITGAV:ITGB3 (PubMed:27993971).
Post-translational Modifications: Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro). The protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.
Similarity: Belongs to the tetraspanin (TM4SF) family.
Immunogen: Purified recombinant fragment of human CD9 expressed in E. Coli
Subcellular Location: Membrane.
Tissue Specificity: Detected in platelets (at protein level). Expressed by a variety of hematopoietic and epithelial cells.