anti-CD95 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD95; anti-CD95 antibody;
The CD95 cd95 (Catalog #MBS8506041) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD95 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF). Researchers should empirically determine the suitability of the CD95 cd95 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The CD95 cd95 product has the following accession number(s) (GI #13539243) (NCBI Accession #CAC35541.1) (Uniprot Accession #Q9UEL0). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
4ADX2 monoclonal antibody reacts with human CD95 (Fas, Apo-1), a 45 kDa member of the TNFR superfamily. CD95 is expressed in different tumor cells and normal cells, and is priorities for CD45RO with strong-memory T cells and g/d T cell.CD95 expression is upregulated by auto-immune disease and virus infection(AIDS), CD178(Fas ligand) binding to CD95 induces apoptosis and has been shown to play a role in the maintenance of peripheral tolerance.
Experimental Methods: 1.Take 100mul peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add 10mul labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1�RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10�);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3]
[Cell fixation: 2% formaldehyde solution]. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD95 are readily searchable from our website. Different antibodies against the same target such as CD95 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Adenocarcinoma, Cardiovascular Diseases, Disease Models, Animal, Drug Toxicity, Inflammation, Liver Diseases, Necrosis, Neoplasms, Nervous System Diseases, Skin Diseases are some of the diseases may be linked to Mouse anti-Human CD95, PE Conjugated mAb.