|The CD98 slc7a5 (Catalog #MBS666505) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD98-Purified Preservative Free reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD98 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD98 slc7a5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD98 slc7a5 product has the following accession number(s) (GI #3767584) (NCBI Accession #BAA33851.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD98-Purified Preservative Free with the following immunoassay(s):
Information: Human CD98 (4F2) is expressed on monocytes and activated cells. All cultured human cell lines tested express CD98. CD98 is thought to be an activation antigen. Antibody UM7F8 recognizes a 125 kd heterodimeric membrane glycoprotein CD98. Antibody UM7F8 is comitogenic with soluble anti-CD2 and immoblized anti-CD3 mAbs.
Performance: Five x 105 cultured Jurkat human tumor cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD98 antibody at 1 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.82 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2u sterile filtered and vialed under aseptic conditions.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl.
Immunogen: Molt 13 T cell line. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD98 are readily searchable from our website. Different antibodies against the same target such as CD98 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Brain, Eye, Intestine, Kidney, Liver, Lung, Lymph, Lymph Node, Muscle tissues are correlated with this protein.