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anti-CDK1 antibody product blog

Posted on 2015-10-10 17:53:15 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; anti-CDK1 antibody; CDK1; p34 cdc-2 nuclear protein;
The CDK1 cdk1 (Catalog #MBS395057) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The p34 cdc-2 nuclear protein reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s p34 cdc-2 nuclear protein can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Frozen/Paraffin, Western Blot (WB). Researchers should empirically determine the suitability of the CDK1 cdk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CDK1 cdk1 product has the following accession number(s) (GI #4502709) (NCBI Accession #NP_001777.1) (Uniprot Accession #P06493). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the p34 cdc-2 nuclear protein with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemical staining of formalin-fixed paraffin-embedded human small intestine tissue after heat induced antigen retrival. Antibody used at 10 ug/ml.)
Immunohistochemistry (IHC) CDK1.

p34(Cdc2) kinase is an an essential nuclear protein for mitosis in human cells. During the prophase/metaphase transition the inactive phosphorylated form of p34(cdc2) is dephosphorylated to make the active form of the protein. This dephosphorylation is performed by the p80(cdc25) dual-specificity phosphatase. The regulatory subunit of p34(cdc2) is cyclin B, a 47-52 kDa protein. The p34(cdc2) protein is the catalytic subunit of the M phase-promoting factor (MPF) which is intracellular factor responsible for entry into M phase. Three mechanisms are responsible for the activitation of MPF at the onset of mitosis: binding of p34(cdc2) to cyclin B, a series of phosphorylation/dephosphorylation events on the two subunits and translocation of the complex to the nucleus or microtubules.

Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant human p34.cdc2 fusion protein and mouse myeloma cells. Positive Control: HeLa cell lysate. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CDK1 are readily searchable from our website. Different antibodies against the same target such as CDK1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. CDK1 also interacts with the following gene(s): BIRC5, BUB1, CCNA1, CCNA2, CCNB1, CCNB2, CCNE1, CDC25C, CDC6, RRM2.
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