anti-CDK4 antibody product blog
Tags: Antibody; Monoclonal Antibody; CDK4; anti-CDK4 antibody;
The CDK4 cdk4 (Catalog #MBS9215557) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CDK4 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s CDK4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).WB ~~ 1:2000. Researchers should empirically determine the suitability of the CDK4 cdk4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CDK4 cdk4 product has the following accession number(s) (GI #4502735) (NCBI Accession #NP_000066.1) (Uniprot Accession #P11802). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CDK4 Antibody with the following immunoassay(s):
Western Blot (WB) (All lanes : Anti-CDK4 Antibody at 1:2000 dilution
Lane 1: A431 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: K562 whole cell lysate
Lane 4: MCF-7 whole cell lysate
Lane 5: NIH/3T3 whole cell lysate
Lysates/proteins at 20 mug per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 34 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.)
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Antigen Source: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CDK4 are readily searchable from our website. Different antibodies against the same target such as CDK4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Adenocarcinoma, Breast Neoplasms, Carcinoma, Cell Transformation, Neoplastic, Liver Diseases, Liver Neoplasms, Lung Neoplasms, Neoplasms, Experimental, Nervous System Diseases, Skin Neoplasms are some of the diseases may be linked to CDK4 Antibody.