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anti-CEACAM8 antibody product blog

Posted on 2015-12-07 09:03:19 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; anti-CEACAM8 antibody; CEACAM8;
The CEACAM8 ceacam8 (Catalog #MBS468020) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CEACAM8 monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CEACAM8 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), CELISA, ELISA (EIA).
Flow Cytometry: 1.2 ug/10^6 cells
CELISA: 1:200 - 1:400. Researchers should empirically determine the suitability of the CEACAM8 ceacam8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CEACAM8 ceacam8 product has the following accession number(s) (GI #21314600) (NCBI Accession #NP_001807.2) (Uniprot Accession #P31997). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CEACAM8 monoclonal antibody with the following immunoassay(s):
Testing Data (Fig.1: FACS analysis of BOSC23 cells using GM2H6 Cat.# GM- 0512. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM8 (red curve) or an irrelevant protein (control transfectant). Binding of GM-0512 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CEACAM8 transfected cells.)
Testing Data CEACAM8.

Testing Data #2 (Fig. 2: BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or PSG. The latter expressed as a membrane bound fusion protein. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1,3,4,5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8: Tet2; PSG: BAP3; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified GM-2H6 was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM8 transfected cells (red curve).)
Testing Data #2 CEACAM8.

Background: CEA-related cell adhesion molecule 8 (CEACAM8, CD66b) belongs to the carcinoembryonic antigen (CEA) gene family (1,3,4). It encodes a glycosylphosphatidylinositol (GPI)-linked glycoprotein with a Mr of 95,000 which is expressed in cells of the granulocyte-lineage. It is expressed in neutrophils and eosinophils and is characterized as a granulocyte-specific activation antigen. CEACAM8 could serve as a marker for granulocyte activities (2). Like all members of the CEA family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by two immunoglobulin constant-like An and B domains.

Buffer: phosphate buffered saline, pH 7.2
Immunogen: genetic immunisation with cDNA encoding human CEACAM8
Selection: based on recognition of the complete native protein expressed on transfected mammalian cells
Immunogen: cDNA. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CEACAM8 are readily searchable from our website. Different antibodies against the same target such as CEACAM8 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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