anti-CGRP antibody product blog
Tags: Antibody; CGRP; Monoclonal Antibody; anti-CGRP antibody; CGRP / Calcitonin;
The CGRP calca (Catalog #MBS249377) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CALCA; Alpha-type CGRP; CGRP; CGRP-I; CGRP1; CT; Calcitonin; KC; CALC1; Calcitonin 1; Katacalcin reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CGRP / Calcitonin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB).IHC - Paraffin: (1:100)
Western blot: (1:100 - 1:250)
Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the CGRP calca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CGRP calca product has the following accession number(s) (GI #4502545) (NCBI Accession #NP_001732.1) (Uniprot Accession #P01258). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CALCA; Alpha-type CGRP; CGRP; CGRP-I; CGRP1; CT; Calcitonin; KC; CALC1; Calcitonin 1; Katacalcin with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-CGRP / Calcitonin antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)
Western Blot (WB) (CALCA/CT Antibody western blot of CEM cell line lysates (35 ug/lane). The CALCA/CT antibody detected the CALCA/CT protein (arrow).)
CALCA Antibody, Alpha-type CGRP Antibody, CGRP Antibody, CGRP-I Antibody, CGRP1 Antibody, CT Antibody, Calcitonin Antibody, KC Antibody, CALC1 Antibody, Calcitonin 1 Antibody, Katacalcin Antibody Description: CALCA encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide.
Immunogen Species: CALCA antibody was raised against Human
Family/Subfamily: Hormone/not assigned-Hormone. Target: CALCA
Epitope: aa56-84
Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:
Tissue Preparation:
Formalin fixation and embedding in paraffin wax.
Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60�C.
Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT
Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT
Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100�C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT
Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply an universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT
Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip.