anti-CHEK2 antibody product blog
Tags: Antibody; Monoclonal Antibody; Chk2; anti-CHEK2 antibody; CHEK2;
The CHEK2 chek2 (Catalog #MBS608357) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Chk2 (BSA & Azide Free) (Checkpoint Kinase 2, Rad 53) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Chk2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF).Dilution: Immunofluorescence
Immunoprecipitation (Native verified) (Use Protein G) (Ab at 2ug/mg protein lysate)
Western Blotting (Ab 1-2ug/ml for 2hrs at RT) The optimal dilution for a specific application should be determined by the investigator. Researchers should empirically determine the suitability of the CHEK2 chek2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CHEK2 chek2 product has the following accession number(s) (GI #11034732) (Uniprot Accession #O96017). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
Not determined.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Chk2 (BSA & Azide Free) (Checkpoint Kinase 2, Rad 53) with the following immunoassay(s):
Western Blot (WB) (Western Blot, human cells)
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both functionas essential components in the G2 DNA damage checkpoint by phophorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.
Immunogen: Recombinant Chk2 protein
Cellular Localization: Nuclear. Positive Control: HeLa cells. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CHEK2 are readily searchable from our website. Different antibodies against the same target such as CHEK2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.