anti-Chk2 antibody product blog
Tags: Antibody; Monoclonal Antibody; Chk2; anti-Chk2 antibody;
The Chk2 chek2 (Catalog #MBS460223) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Chk2 Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Chk2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB).ELISA: 0.01-0.1 ug/ml
Flow Cytometry: 5-10 ug/ml
Immunohistochemistry: 2-10 ug/ml
Immunoprecipitation: 2-5 ug/ml
Western Blot: 0.1-1 ug/ml. Researchers should empirically determine the suitability of the Chk2 chek2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Chk2 chek2 product has the following accession number(s) (GI #11034732) (NCBI Accession #BAB17231.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. Both Chk1 and Chk2 contain a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1. In addition, CHKs interact with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in CHK2 gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants (a, b, c) encoding different isoforms have been found.
Preparation: Purified recombinant CHK2 protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Chk2 are readily searchable from our website. Different antibodies against the same target such as Chk2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.