anti-CIRE antibody product blog
Tags: Antibody; Monoclonal Antibody; CIRE; anti-CIRE antibody;
The CIRE n/a (Catalog #MBS832110) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CIRE can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS).FC: <= 0.5 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the CIRE n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CIRE antibody (PE) with the following immunoassay(s):
Flow Cytometry (FC/FACS) (Staining of mouse CD209-transfected CHO cells with 0.25 ug of Rat IgG2a kappa Isotype Control (PE) (open histogram) or 0.25 ug of CD209 antibody (PE) (filled histogram). Total viable cells were used for analysis.)
Rat monoclonal CIRE antibody (PE).
Biological Significance: The LWC06 antibody was generated by immunization with the recombinant extracellular region of mouse CIRE/DC-SIGN. CIRE/DC-SIGN was identified by its expression on CD8 alpha- dendritic cells and plasmacytoid predendritic cells, and is the closest homologue of human DC-SIGN. Human DC-SIGN was originally identified in human placenta for its ability to bind the HIV envelope protein gp120 in a CD4-independent manner. CIRE/DC-SIGN is a 33 kDa type II transmembrane C-type lectin protein. It contains a C-terminal, extracellular, Carbohydrate Recognition Domain that is predicted to bind mannose and other carbohydrates in a Ca2+ dependent manner. It has been postulated that CIRE/DC-SIGN may play a role in T-dendritic cell interactions through binding with members of the ICAM family. CIRE/DC-SIGN is differentially expressed by sub-populations of dendritic cells and preliminary data suggest that its expression varies depending on the activation state of the host. CIRE/DC-SIGN is down-regulated in spleen-derived dendritic cell cultures supplemented with GM-CSF. While human DC-SIGN is predominantly expressed in dendritic cells, CIRE/DC-SIGN mRNA has also been detected in B cells. The LWC06 monoclonal antibody does not cross-react with the closely related SIGNR1, SIGNR2, SIGNR3 or SIGNR4. Tag/Conjugate: PE. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CIRE are readily searchable from our website. Different antibodies against the same target such as CIRE may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.