anti-CRB3 antibody product blog
Tags: Antibody; Monoclonal Antibody; CRB3; anti-CRB3 antibody;
The CRB3 crb3 (Catalog #MBS588104) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The CRB3 Rat Monoclonal Antibody (14F9) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s CRB3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).WB 1:1000
IHC: 1:100
IF: 1:100
Optimal dilution has to be determined by the user. Researchers should empirically determine the suitability of the CRB3 crb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CRB3 crb3 product has the following accession number(s) (GI #37182490) (NCBI Accession #AAQ89047.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CRB3 Rat Monoclonal Antibody (14F9) with the following immunoassay(s):
Western Blot (WB) (LEFT: Western blot analysis shows that antibody recognize the lull length CRB3 (GST-CRB3) but not the extracellular domain of CRBJ (GST-eRB3extra) (anti-CRB3 (14F9) was used at 1:1000) RIGHT: Western blot analysis shows that antibody recognize overexpressed (HEK293T cells) and endogenous CRB3 (mouse tissues). (antiCRB3 (14F9) was used at 1:1000).)
Immunohistochemistry (IHC) (TOP: Immunofluorescence analysis of over expressed human CRB3 In Iysates of HeLa cells. Bottom: Immunofluorescence analysis of endogenous CRB3 expression in the mouse Intestine. OAPI was used to stain the nuclei In HeLa cells. where as E-cadherin staining was used to highlight the lateral membrane in mouse intestine.)
CRB3 (Crumbs homologue 3) is an apical transmembrane protein member of the CRB protein family reported to be associated with tight junctions at the apical surface of epithelial cells and to play a role in apical membrane morphogenesis and tight junction regulation. The formation and maintenance of tight junctions is essential for the development of epithelial cell polarity. Two CRB3 isoforms have been described and CRB3 is homologous to the Crumb protein found in Drosophila. CRB3 contains a putative signal peptide in its first 20 amino acids, a putative extracellular site, and a transmembrane domain. The intracellular domain contains a PDZ-binding motif which is involved in the interactions of CRB3 with PARD6A and MPP5. Loss of CRB3 expression in immortal baby mouse kidney (iBMK) cells was reported to render these cells tumorigenic, suggesting an important role for CRB3 function in maintaining epithelial junction formation and apicobasal polarity required for contact-inhibited growth, and to suppress invasion and metastasis.
Immunogen: Full length protein
Target: CRB3. Preservative: None. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CRB3 are readily searchable from our website. Different antibodies against the same target such as CRB3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.