anti-CRYAB antibody product blog
Tags: Antibody; CRYAB; Monoclonal Antibody; Alpha B Crystallin; anti-CRYAB antibody;
The CRYAB cryab (Catalog #MBS800595) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Alpha B Crystallin Antibody: ATTO 390 reacts with Human, Bovine and may cross-react with other species as described in the data sheet. MyBioSource\'s alpha B Crystallin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA).1:2000 (WB). Researchers should empirically determine the suitability of the CRYAB cryab for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CRYAB cryab product has the following accession number(s) (GI #4503057) (NCBI Accession #NP_001876.1) (Uniprot Accession #P02511). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Alpha B Crystallin Antibody: ATTO 390 with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Bovine cell lysates showing detection of Alpha B Crystallin protein using Mouse Anti-Alpha B Crystallin Monoclonal Antibody, Clone 1A7.D5. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody at 1:50 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Background Info: Detects a band at ~20kDa (Predicted mol. weight is ~21kDa) corresponding to alphaB-crystallin. Does not cross-react with alphaA-crystallin, betaL-crystallin, betaH-crystallin, gamma-crystallin, Hsp25, Hsp27 or Hsp47 proteins.
Scientific Background: The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with Hsp25 and Hsp27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-An and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its\' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-An and B prevent apoptosis by inhibiting caspases (8). Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions (9).