anti-CytokeRatin 7 / KeRatin K7 antibody product blog
Tags: Antibody; Monoclonal Antibody; CytokeRatin 7 / KeRatin K7; anti-CytokeRatin 7 / KeRatin K7 antibody;
The CytokeRatin 7 / KeRatin K7 n/a (Catalog #MBS570002) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti CytokeRatin 7 / KeRatin K7 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CytokeRatin 7 / KeRatin K7 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB).OVTL12/30 is effective on frozen tissue after fixation in methanol. It is particularly effective on paraffin-embedded tissue, for which pretreatment with e.g. 0.1% pronase / 0.1% trypsine in PBS or 0.5% pepsine in 0.01N HCl or microwave pretreatment is required. In addition, OVTL 12/30 is suitable for immunocytochemistry, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations. Researchers should empirically determine the suitability of the CytokeRatin 7 / KeRatin K7 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse anti CytokeRatin 7 / KeRatin K7 with the following immunoassay(s):
Immunohistochemistry (IHC) (Figure 2. Immunohistochemistry on paraffin section of human liver. Bile ducts are strongly positive.)
Immunohistochemistry (IHC) (Figure 3. Immunohistochemistry on paraffin section of human breast. Positive staining of lobuli and ducti.)
Immunohistochemistry (IHC) (Figure 4: Frozen section of human kidney tissue immunostained showing strong reactivity in the epithelial cells of the tubules.)
CytokeRatins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual Human cytokeRatins are numbered 1 to 20. The various epithelia in the Human body usually express cytokeRatins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. CytokeRatin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeRatin antibodies are not only of assistance in the differential identification of tumors using immunohistochemistry on tissue sections, but are also an useful tool in cytopathology and flow cytometric assays.
Source Note: OVTL12/30 is a Mouse monoclonal IgG1/kappa antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Mouse immunized with a cytoskeletal prepaRation of OTN-11 ovarian carcinoma cell line. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CytokeRatin 7 / KeRatin K7 are readily searchable from our website. Different antibodies against the same target such as CytokeRatin 7 / KeRatin K7 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.