anti-DAN antibody product blog
Tags: Antibody; Monoclonal Antibody; DAN; anti-DAN antibody;
The DAN nbl1 (Catalog #MBS692409) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse DAN reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s DAN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the DAN nbl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The DAN nbl1 product has the following accession number(s) (GI #148234601) (NCBI Accession #NP_032701.2) (Uniprot Accession #Q61477). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
DAN (differential screening-selected gene aberrative in neuroblastoma) was initially identified as a gene whose expression is downregulated in srctransformed rat fibroblasts. DAN has now been shown to be a prototypical member of the DAN family of secreted glycoproteins that are putative BMP antagonists. DAN family members share a cysteinerich domain that is structurally related to the cysteineknot motif found in TGFbeta superfamily ligands. There are at least five mouse DAN family members, including DAN, Gremlin/DRM, Cer1 (Cerberusrelated), Dante and PRDC (protein-related to DAN and cerberus). Additional DAN family members include Xenopus Cerberus, chick Caronte, and C. elegans CeCan 1. Murine DAN binds BMP2 in immunoprecipitation experiments and acts as a BMP antagonist in Xenopus animal cap explants. The DAN family of proteins are thought to act as antagonists by binding BMP ligands and preventing their interactions with signaling receptor complexes. Recombinant mouse DAN have been shown to bind BMP4 in a functional ELISA. It is likely the various DAN family members and other TGFbeta BMP antagonists including Noggin, Chordin, Follistatin, and TSG can selectively antagonize the activities of different subsets of TGFbeta superfamily ligands. These antagonists represent one of the many elaborate regulatory mechanisms that have evolved to control the bioactivities of the TGFbeta superfamily ligands.
Reconstitution: Reconstitute the antibody with 500 ul sterile PBS and the final concentration is 200 ug/ml. Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant DAN. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing DAN are readily searchable from our website. Different antibodies against the same target such as DAN may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.