anti-DECTIN-1 antibody product blog
Tags: Antibody; Monoclonal Antibody; DECTIN-1; anti-DECTIN-1 antibody;
The DECTIN-1 clec7a (Catalog #MBS219635) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s DECTIN-1 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS).Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10. Researchers should empirically determine the suitability of the DECTIN-1 clec7a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The DECTIN-1 clec7a product has the following accession number(s) (GI #13384604) (NCBI Accession #NP_072092.2) (Uniprot Accession #Q9BXN2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI HUMAN DECTIN-1:FITC with the following immunoassay(s):
Testing Data (Staining of human peripheral blood monocytes wtih Mouse anti Human Dectin:RPE (MBS219691))
Testing Data (Staining of human peripheral blood monocytes wtih Mouse anti Human Dectin:Alexa Fluor ?488 (MBS219608A488))
Testing Data (Staining of human peripheral blood monocytes wtih Mouse anti Human Dectin (MBS219608))
Testing Data (Staining of human peripheral blood monocytes wtih Mouse anti Human Dectin:FITC (MBS219393))
Mouse anti Human Dectin-1 antibody, clone GE2 recognizes human Dectin-1, also known as beta-glucan receptor (betaGR), a C-type lectin domain family member. It is a major pathogen pattern-recognition receptor that binds beta-glucans, glucose polymers that form cell-wall components or exopolymers of yeasts, fungi and some bacteria. Beta-glucans have also been used experimentally and therapeutically as immunomodulators that enhance resistance to bacterial, yeast, viral and protozoan infections, as well as tumor formation. Dectin-1 also acts as a co-stimulatory molecule on T-cells to induce their proliferation and is necessary for the TLR2-mediated inflammatory response. It is highly expressed on peripheral blood leukocytes and dendritic cells. Human Dectin-1 has two major (betaGR-An and betaGR-B) and several minor isoforms. betaGR-An and betaGR-B differs by the presence and absence, respectively, of a stalk region. Mouse anti Human Dectin-1 antibody, clone GE2recognizes both of these isoforms. Clone GE2 could be used to inhibit receptor function.
Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Immunogen: NIH3T3 cells expressing full length Beta-glucan receptor.
Fusion Partners: Spleen cells from immunised C57BL/6 mice were fused with cells of the murine SP2 myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing DECTIN-1 are readily searchable from our website. Different antibodies against the same target such as DECTIN-1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.