|The Dendritic Cell n/a (Catalog #MBS834112) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Dendritic Cell can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB).
FC: <= 0.25 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest. Researchers should empirically determine the suitability of the Dendritic Cell n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Dendritic Cell antibody with the following immunoassay(s):
Flow Cytometry (FC/FACS) (Staining of BALB/c splenocytes with 0.25 ug of Rat IgG2b kappa Isotype Control (open histogram) or 0.25 ug of Dendritic Cell Marker antibody (filled histogram) followed by Anti-Rat IgG (biotin) and Streptavidin (PE). Total viable cells were used for analysis.)
Rat monoclonal Dendritic Cell antibody.
Biological Significance: The 33D1 monoclonal antibody reacts with a mouse DC-specific surface marker. The nature and biological activity of the 33D1 antigen is as of yet unknown. 33D1 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer?s patch. Bone marrow dendritic cells require GM-CSF to express the 33D1 antigen and this expression is down regulated in the presence of IL-4. 33D1 antigen has been detected in vivo in brain dendritic cells post infection via Toxoplasma gondii. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Dendritic Cell are readily searchable from our website. Different antibodies against the same target such as Dendritic Cell may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.