anti-Dibromo-tyrosine antibody product blog
Tags: Antibody; Monoclonal Antibody; Dibromo-tyrosine; anti-Dibromo-tyrosine antibody;
The Dibromo-tyrosine n/a (Catalog #MBS808626) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Dibromo-tyrosine Antibody, Clone 6G3: RPE reacts with Species Independent and may cross-react with other species as described in the data sheet. MyBioSource\'s Dibromo-tyrosine can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA).WB: 1:1000
ICC/IF: 1:50
ELISA: 1:1000. Researchers should empirically determine the suitability of the Dibromo-tyrosine n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Dibromo-tyrosine Antibody, Clone 6G3: RPE with the following immunoassay(s):
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 6G3. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Dibromo-tyrosine Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Dot Blot (DB) (Dot Blot analysis using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 6G3. Tissue: 3, 5-Dibromotyrosine-BSA Conjugate. Species: Species Independent. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:4000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.)
Western Blot (WB) (Western Blot analysis of 3, 5-Dibromotyrosine-BSA Conjugate showing detection of 67 kDa Dibromo-tyrosine protein using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 6G3. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: Nitrosylated-BSA. Lane 4: Dibromotyrosine-BSA. Load: 1 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.)
Western Blot (WB) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Dibromo-tyrosine protein using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 6G3. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.)
Target: Dibromo-tyrosine
Immunogen: Synthetic 3,5-Dibromotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate: RPE
Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide.