|The Dlg2 dlg2 (Catalog #MBS803343) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Chapsyn-110 Antibody: APC reacts with Human (weak), Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Chapsyn-110 can be used in a range of immunoassay formats including, but not limited to, Western (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP).
1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF). Researchers should empirically determine the suitability of the Dlg2 dlg2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Dlg2 dlg2 product has the following accession number(s) (GI #11560113) (NCBI Accession #NP_071618.1) (Uniprot Accession #Q63622). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Chapsyn-110 Antibody: APC with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Rat brain membrane lysate showing detection of Chapsyn-110 protein using Mouse Anti-Chapsyn-110 Monoclonal Antibody, Clone S18-30. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Chapsyn-110 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Background Info: Detects ~110kDa. No cross-reactivity against other MAGUK family members, PSD95, SAP97, SAP102, expressed in transfected cells.
Scientific Background: Chapsyn-110, is a part of the membrane-associated putative guanylate kinase (MAGUK) family. It binds directly to the NMDA receptor, and Shaker K+ channel subunits, and is 70-80% identical to PSD-95/SAP90 and SAP97 (1). It associated tightly with the postsynaptic density I brain, and mediates the clustering of both NMDA recpetors and K+ channels in heterologous cells. The encoded protein forms a heterodimer with PSD-95 that may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins (1, 2).
Conjugate: APC. Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Dlg2 are readily searchable from our website. Different antibodies against the same target such as Dlg2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.