Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Monoclonal Antibody Blog  rightarrow anti-Dlg4 antibody  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • anti-S100A8 antibody blog
 • anti-EPM2A antibody blog
 • anti-CD33 antibody blog
 • anti-HE4 antibody blog
 • anti-TUBB antibody blog
 • anti-IgG (Fc) antibody blog
 • anti-CNTN1 antibody blog
 • anti-MHC Class 1, H2, Dk antibody blog
 • anti-CFLAR antibody blog
 • anti-TNF-a antibody blog
 • anti-HPGD antibody blog
 • anti-PDGFRA antibody blog
 • anti-UBC antibody blog
 • anti-NT5E antibody blog
 • anti-Cortisol antibody blog
 • anti-Cytokeratin 5 & 14 & p63 antibody blog
 • anti-CD16/CD32 antibody blog
 • anti-PNMT antibody blog
 • anti-CD98 antibody blog
 • anti-SLC5A5 antibody blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Glutamate carboxypeptidase 2 (FOLH1) ELISA Kit
 • Advanced oxidation protein products (AOPP) ELISA Kit
 • 11 beta hydroxysteroid dehydrogenase type 1 (11beta HSD1) (H... ELISA Kit
 • Phosphorylation Extracellular Signal Regulated Kinase 1-2 (P... ELISA Kit
 • sCD23 ELISA Kit
 • GFP Antibody
 • thyroxine, T4 ELISA Kit
 • Advanced Glycosylation End Products (AGES) ELISA Kit
 • ACAA1 Antibody
 • O-acetylhomoserine (thiol)-lyase (SPBC428.11) Recombinant Protein
 • KCNMA1 Antibody
 • 3-Hydroxy-3-methylglutaryl CoA reductase (HMG-CoA) ELISA Kit
 • MHC class I chain-related gene A (MICA) Recombinant Protein
 • T4 (Thyroxine) (THVgp004) Antibody
 downarrow more ...
separator

anti-Dlg4 antibody product blog

separator
Posted on 2016-01-21 07:49:46 by mybiosource_staff
Share it on ...     
separator
separator
Tags: Antibody; DLG4; Monoclonal Antibody; PSD95; anti-DLG4 antibody;
separator
The Dlg4 dlg4 (Catalog #MBS800387) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PSD95 Antibody: ATTO 594 reacts with Mouse, Rat, Bovine, Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PSD95 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC).
1:250 (WB). Researchers should empirically determine the suitability of the Dlg4 dlg4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The Dlg4 dlg4 product has the following accession number(s) (GI #9665227) (NCBI Accession #NP_062567.1) (Uniprot Accession #P31016). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PSD95 Antibody: ATTO 594 with the following immunoassay(s):
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 6G6. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Junction staining.)
Immunocytochemistry/Immunofluorescence (ICC/IF) Dlg4.

Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 6G6. Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.)
Immunocytochemistry/Immunofluorescence (ICC/IF) Dlg4.

Western Blot (WB) (Western Blot analysis of Rat brain membrane lysate showing detection of PSD95 protein using Mouse Anti-PSD95 Monoclonal Antibody, Clone 6G6. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000.)
Western Blot (WB) Dlg4.

Background Info: Detects a ~100kDa protein corresponding to the molecular mass of PSD-95 on SDS PAGE immunoblots. An additional protein of >100kDa is also detected. Additional cross-reactive bands are detected at ~75kDa and 50kDa in rat and mouse samples.

Scientific Background: Postsynaptic Density protein 95 (PSD95), also known as Synapse associated protein 90kDa, is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex (1). These family members consist of an N-terminal variable segment followed by three amino-terminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain. The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins which are neuronal cell adhesion molecules that interact with b-neurexins and form intercellular junctions. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins (2). Thus different PDZ domains of PSD-95 might be specialized for distinct functions (3, 4). PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (5). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning (6). Mutations in PSD95 are associated with autism (7).

horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions