|The Dlg4 dlg4 (Catalog #MBS801790) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PSD95 Antibody: ATTO 680 reacts with Mouse, Rat, Bovine, Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PSD95 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC).
1ug/ml was sufficient for detection of PSD-95 on 20ug rat brain tissue extract. Researchers should empirically determine the suitability of the Dlg4 dlg4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Dlg4 dlg4 product has the following accession number(s) (GI #9665227) (NCBI Accession #NP_062567.1) (Uniprot Accession #P31016). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PSD95 Antibody: ATTO 680 with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3. Tissue: Neocortex. Species: Rat. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000.)
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filamentous-like staining.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3. Tissue: backskin. Species: Mouse. Fixation: Bouin\'s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Basal cell staining in the epidermis, some hair follicle staining, dermal staining. Backskin obtained from transgenic mice.)
Western Blot (WB) (Western Blot analysis of Rat brain membrane lysate showing detection of PSD95 protein using Mouse Anti-PSD95 Monoclonal Antibody, Clone 7E3. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000.)
Background Info: Detects a ~100kDa protein corresponding to the molecular mass of PSD-95 on SDS PAGE immunoblots. Additional cross-reactive bands are detected at ~80kDa and ~50kDa in rat and mouse samples.
Scientific Background: Postsynaptic Density protein 95 (PSD95), also known as Synapse associated protein 90kDa, is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex (1). These family members consist of an N-terminal variable segment followed by three amino-terminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain. The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins which are neuronal cell adhesion molecules that interact with b-neurexins and form intercellular junctions. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins (2). Thus different PDZ domains of PSD-95 might be specialized for distinct functions (3, 4). PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (5). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning (6). Mutations in PSD95 are associated with autism (7).