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anti-Dlg4 antibody product blog

Posted on 2015-12-22 18:32:56 by mybiosource_staff
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Tags: Antibody; DLG4; Monoclonal Antibody; PSD95; anti-DLG4 antibody;
The Dlg4 dlg4 (Catalog #MBS803784) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PSD95 Antibody: FITC reacts with Mouse, Rat, Bovine, Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PSD95 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC).
1ug/ml was sufficient for detection of PSD-95 on 20ug rat brain tissue extract. Researchers should empirically determine the suitability of the Dlg4 dlg4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The Dlg4 dlg4 product has the following accession number(s) (GI #9665227) (NCBI Accession #NP_062567.1) (Uniprot Accession #P31016). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PSD95 Antibody: FITC with the following immunoassay(s):
Testing Data (PSD95 visualized.
Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.)
Testing Data Dlg4.

Testing Data #2 (ICC localization of Psd95 in rat neocortex.)
Testing Data #2 Dlg4.

Testing Data #3 (Western blot analysis of PSD95 in rat membrane using a 1:1000 dilution.)
Testing Data #3 Dlg4.

Background Info: Detects a ~100kDa protein corresponding to the molecular mass of PSD-95 on SDS PAGE immunoblots. Additional cross-reactive bands are detected at ~80kDa and ~50kDa in rat and mouse samples.

Scientific Background: Postsynaptic Density protein 95 (PSD95), also known as Synapse associated protein 90kDa, is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex (1). These family members consist of an N-terminal variable segment followed by three amino-terminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain. The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins which are neuronal cell adhesion molecules that interact with b-neurexins and form intercellular junctions. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins (2). Thus different PDZ domains of PSD-95 might be specialized for distinct functions (3, 4). PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (5). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning (6). Mutations in PSD95 are associated with autism (7).

Conjugate: FITC. Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Dlg4 are readily searchable from our website. Different antibodies against the same target such as Dlg4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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