|The DMPO dmpo (Catalog #MBS802000) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DMPO Antibody: FITC reacts with Species Independent and may cross-react with other species as described in the data sheet. MyBioSource\'s DMPO can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the DMPO dmpo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The DMPO dmpo product has the following accession number(s) (GI #51242284) (NCBI Accession #AAT99086.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the DMPO Antibody: FITC with the following immunoassay(s):
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DMPO Monoclonal Antibody, Clone N1664A. Tissue: macrophage cell line (Raw 264.7). Species: Mouse. Primary Antibody: Mouse Anti-DMPO Monoclonal Antibody at 1:100. Secondary Antibody: Alexa Fluor 488 Goat Anti-Mouse (green) at 1:1000. Counterstain: DAPI (blue) nuclear stain.)
Western Blot (WB) (Western Blot analysis of Human HL 60 clone 15 eosinophils lysates showing detection of DMPO protein using Mouse Anti-DMPO Monoclonal Antibody, Clone N1664A. Primary Antibody: Mouse Anti-DMPO Monoclonal Antibody at 1:200.)
Background Info: Detects ~90kDa. Recognzies DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.
Scientific Background: The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states (1). The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems (2-6). Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals (1). DMPO adducts can be generated with protein and DNA radicals (7).
Certificate of Analysis: A 1/1000 dilution was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100ng/lane by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Conjugate: FITC. Storage Buffer: TBS pH7.4, 0.05% sodium azide, 50% glycerol. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing DMPO are readily searchable from our website. Different antibodies against the same target such as DMPO may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.