anti-DNA/RNA Damage antibody product blog
Tags: Antibody; Monoclonal Antibody; DNA/RNA Damage; anti-DNA/RNA Damage antibody;
The DNA/RNA Damage n/a (Catalog #MBS802475) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DNA/RNA Damage Antibody, 15A3; Anti-DNA/RNA Damage Antibody reacts with All and may cross-react with other species as described in the data sheet. MyBioSource\'s DNA/RNA Damage can be used in a range of immunoassay formats including, but not limited to, Immunohistochemitsry (IHC), Immunocytochemistry (ICC), Immunofluroescence (IF), ELISA, Dot Blot (DB), Immunoprecipitation (IP), Flow Cytometry (FCM), Flow Assay (FA).IHC: (1:1000); optimal dilutions for assays should be determined by end user. Researchers should empirically determine the suitability of the DNA/RNA Damage n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the DNA/RNA Damage Antibody, 15A3; Anti-DNA/RNA Damage Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-DNA Damage Monoclonal Antibody, Clone 15A3. Tissue: Retinal Injury Model. Species: Mouse. Primary Antibody: Mouse Anti-DNA Damage Monoclonal Antibody at 1:1000. Secondary Antibody: Alexa Fluor 594 Goat Anti-Mouse (red). Courtesy of: Dr. Rajashekhar Gangaraju, University of Indiana, Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-DNA Damage Monoclonal Antibody, Clone 15A3. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-DNA Damage Monoclonal Antibody at 1:1000000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x. With anti-microbial.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-DNA Damage Monoclonal Antibody, Clone 15A3. Tissue: backskin. Species: Mouse. Fixation: Bouin\'s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-DNA Damage Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-DNA Damage Monoclonal Antibody, Clone 15A3. Tissue: Ischemic fresh brain tissue. Species: Rat. Primary Antibody: Mouse Anti-DNA Damage Monoclonal Antibody at 1:1000 for 16 hours at RT. Secondary Antibody: Alexa Fluor 546 Goat Anti-mouse (Red) at 1:500 for 1 hour at RT. Localization: Cerebral Cortex. Courtesy of: Dr. Yi Yang, U. New Mexico.)
Scientific Background: DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are oxidation, alkylation, hydrolysis and mismatch of the bases. During the oxidation of bases, highly reactive chemical entities collectively known as RONS, occurs. RONS stands for reactive oxygen and nitrogen species and includes nitric oxide, superoxide, hydroxyl radical, hydrogen peroxide and peroxynitrite. Numerous studies have shown that RONS causes a variety of issues including DNA damage (1). 8-hydroxyguanine, 8-hydroxy-2\'-deoxyguanonsine and 8- hydroxyguanosine are all RNA and DNA markers of oxidative damage. 8-hydroxy-2\'-guanosine is produced by reactive oxygen and nitrogen species including hydroxyl radical and peroxynitrite. Specifically its high biological relevance is due to its ability to induce G to T transversions, which is one of the most frequent somatic mutations (2). 8-hydroxy-guanine has been the most frequently studied type of DNA base damage, with studies in diabetes, and cancer. Base modifications of this type arise from radical-induced hydroxylation and cleavage reactions of the purine ring (3, 4). And finally, 8-hydroxy-guanosine, like 8-hydroxy-2\'-guanosine, induces a mutagenic transversion of G to T in DNA. Its role has specifically been tested in the development of diabetes, hypertension and strokes (5, 6, and 7).