|The DNMT1 dnmt1 (Catalog #MBS807040) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human DNMT1 Monoclonal IgG1 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s DNMT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).
Western Blot: 2-4 ug/mL
Immunohistochemistry: 1-2 ug/mL. Researchers should empirically determine the suitability of the DNMT1 dnmt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The DNMT1 dnmt1 product has the following accession number(s) (GI #4503351) (NCBI Accession #NP_001370) (Uniprot Accession #P26358). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).
Immunogen: Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1
Immunogen Species: Human. COA: 2 ug/ml was sufficient for detection of Dnmt1 in 10 ug of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Storage Buffer: PBS, 0.05% BSA, 0.05% sodium azide, 50% glycerol
Cellular Localization: Nucleus. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing DNMT1 are readily searchable from our website. Different antibodies against the same target such as DNMT1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.