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anti-DNMT3A antibody product blog

Posted on 2017-03-19 19:18:11 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; DNMT3A; anti-Dnmt3a antibody;
The DNMT3A dnmt3a (Catalog #MBS668324) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to DNMT3a (Clone: ABM13G4) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s DNMT3a can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB).
WB: 2-4 ug/ml
Flow Cytometry: 0.5-1 ug/10^6. Researchers should empirically determine the suitability of the DNMT3A dnmt3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The DNMT3A dnmt3a product has the following accession number(s) (GI #166215081) (NCBI Accession #Q9Y6K1.4) (Uniprot Accession #Q9Y6K1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Monoclonal Antibody to DNMT3a (Clone: ABM13G4) with the following immunoassay(s):
Western Blot (WB) (Fig-1: Western blot analysis of DNMT3a. Anti-DNMT3a antibody (Clone: ABM13G4) was used at 2 ug/ml on h heart lysate.)
Western Blot (WB) DNMT3A.

Flow Cytometry (FC/FACS) (Fig-2: Intracellular flow cytometric analysis of Dnmt3a on HEK293 cells using 0.5 ug/10^6 cells of antibody (Clone: ABM13G4). Green represents isotype control; red represents anti-Dnmt3a antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
Flow Cytometry (FC/FACS) DNMT3A.

Dnmt3a belongs to the mammalian methyltransferase gene family which is responsible for tissue-specific gene expression. Dnmt3a together with other methyltransferases conducts de novo methylation of cytosine residues in CpG islands by the enzymatic addition of methyl residues from S-adenosyl-L-methionine to the 5-carbon atom of the cytosine ring. Dnmt3a also directs a metabolic program by repressing key genes to enable the coupling of insulin secretion to glucose levels during beta cell maturation. Loss of Dnmt3a in pancreatic beta cells prevents this developmental metabolic reprogramming, resulting in loss of GSIS (Glucose-Stimulated Insulin Secretion)). Dnmt3a overexpression has been found in several cancers including AML (Acute Myeloid Leukemia).

Immunogen Information: A partial length recombinant protein from DNMT3a was used as the immunogen for this antibody.
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