Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Monoclonal Antibody Blog  rightarrow anti-DNMT3A antibody  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • anti-Mfn2 antibody blog
 • anti-CGB8 antibody blog
 • anti-Nhe1 antibody blog
 • anti-DR3 antibody blog
 • anti-TGFa antibody blog
 • anti-WNT3A antibody blog
 • anti-PA2140 antibody blog
 • anti-MATN1 antibody blog
 • anti-CDX2 antibody blog
 • anti-CEACAM5 antibody blog
 • anti-MSTN antibody blog
 • anti-Kcnip3 antibody blog
 • anti-ENO1 antibody blog
 • anti-CD17 antibody blog
 • anti-ADAM17 antibody blog
 • anti-CD11b antibody blog
 • anti-CD147 antibody blog
 • anti-DLX4 antibody blog
 • anti-TNF alpha antibody blog
 • anti-LOC406081 antibody blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • Testosterone, T ELISA Kit
 • Krebs Von Den Lungen 6 (KL-6) ELISA Kit
 • CD20 - MS4A1 Antibody
 • alpha Lactalbumin (alpha-La) ELISA Kit
 • Reactive oxygen species modulator 1 (ROMO1) ELISA Kit
 • Fibroblast Growth Factor 2 (FGF-2) ELISA Kit
 • ATG16L1 Antibody
 • KRT71 Antibody
 • Bt Cry3B Native Protein
 • fibroblast growth factor receptor 4 (FGFR4) ELISA Kit
 • transcription factor AP-2 epsilon (activating enhancer bindi... ELISA Kit
 • AMPK alpha 1 Antibody
 downarrow more ...

anti-DNMT3A antibody product blog

Posted on 2017-03-19 19:18:11 by mybiosource_staff
Share it on ...     
Tags: Antibody; Monoclonal Antibody; DNMT3A; anti-Dnmt3a antibody;
The DNMT3A dnmt3a (Catalog #MBS668324) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to DNMT3a (Clone: ABM13G4) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s DNMT3a can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB).
WB: 2-4 ug/ml
Flow Cytometry: 0.5-1 ug/10^6. Researchers should empirically determine the suitability of the DNMT3A dnmt3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The DNMT3A dnmt3a product has the following accession number(s) (GI #166215081) (NCBI Accession #Q9Y6K1.4) (Uniprot Accession #Q9Y6K1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Monoclonal Antibody to DNMT3a (Clone: ABM13G4) with the following immunoassay(s):
Western Blot (WB) (Fig-1: Western blot analysis of DNMT3a. Anti-DNMT3a antibody (Clone: ABM13G4) was used at 2 ug/ml on h heart lysate.)
Western Blot (WB) DNMT3A.

Flow Cytometry (FC/FACS) (Fig-2: Intracellular flow cytometric analysis of Dnmt3a on HEK293 cells using 0.5 ug/10^6 cells of antibody (Clone: ABM13G4). Green represents isotype control; red represents anti-Dnmt3a antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
Flow Cytometry (FC/FACS) DNMT3A.

Dnmt3a belongs to the mammalian methyltransferase gene family which is responsible for tissue-specific gene expression. Dnmt3a together with other methyltransferases conducts de novo methylation of cytosine residues in CpG islands by the enzymatic addition of methyl residues from S-adenosyl-L-methionine to the 5-carbon atom of the cytosine ring. Dnmt3a also directs a metabolic program by repressing key genes to enable the coupling of insulin secretion to glucose levels during beta cell maturation. Loss of Dnmt3a in pancreatic beta cells prevents this developmental metabolic reprogramming, resulting in loss of GSIS (Glucose-Stimulated Insulin Secretion)). Dnmt3a overexpression has been found in several cancers including AML (Acute Myeloid Leukemia).

Immunogen Information: A partial length recombinant protein from DNMT3a was used as the immunogen for this antibody.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions