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anti-DNMT3L antibody product blog

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Posted on 2015-12-29 18:20:01 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; DNMT3L; anti-DNMT3L antibody;
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The DNMT3L dnmt3l (Catalog #MBS801970) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Dnmt3L Antibody: Streptavidin reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Dnmt3L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP).
1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF). Researchers should empirically determine the suitability of the DNMT3L dnmt3l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The DNMT3L dnmt3l product has the following accession number(s) (GI #28872778) (NCBI Accession #NP_037501.2) (Uniprot Accession #Q9UJW3). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Dnmt3L Antibody: Streptavidin with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Dnmt3L Monoclonal Antibody, Clone S117-9. Tissue: backskin. Species: Mouse. Fixation: Bouin\'s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Dnmt3L Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Lower layers of the epidermis have positive cell staining in the cytoplasm.)
Immunohistochemistry (IHC) DNMT3L.

Western Blot (WB) (Western Blot analysis of Human tissue lysate showing detection of Dnmt3L protein using Mouse Anti-Dnmt3L Monoclonal Antibody, Clone S117-9. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Dnmt3L Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Western Blot (WB) DNMT3L.

Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dnmt3L Monoclonal Antibody, Clone S117-9. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-Dnmt3L Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Staining in the nucleus, and accumulated around the nucleus. Clear nuclear demarcations.)
Immunocytochemistry/Immunofluorescence (ICC/IF) DNMT3L.

Background Info: Detects ~45kDa. No cross-reactivity against DNMT3A or DNMT3B.

Scientific Background: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue (1-6). Studies show that DNMT3L regulates the activity of DNMT3A and DNMT3B and stimulates their catalytic activities (7). DNMT3L has specifically been linked to the process of carcinogenesis, thru its role in nuclear programming (8).

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