anti-DSG2 antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-DSG2 antibody; DSG2; Desmoglein-2;
The DSG2 dsg2 (Catalog #MBS584040) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Desmoglein-2, Human, mAb 6D8 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Desmoglein-2 can be used in a range of immunoassay formats including, but not limited to, F, Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot.Application Notes: W: A reduced sample treatment (Laemmli buffer) and SDS-PAGE was used. The band size is ~165 kDa (Ref.5).
Application Use: For immune fluorescence and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. Researchers should empirically determine the suitability of the DSG2 dsg2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The DSG2 dsg2 product has the following accession number(s) (GI #116534898) (NCBI Accession #NP_001934.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Product Description: The monoclonal antibody 6D8 recognizes the extracellular domain of human desmoglein-2. Desmogleins and desmocollins are members of the cadherin family of transmembrane proteins that together make up the core of the desmosome, a structure that provides transmembrane strength to tissues undergoing mechanical stress. The desmosomal cadherins, desmogleins and desmocollins, mediate calcium-dependent cell-cell adhesion by forming homotypic and heterotypic interactions with one another. The multiprotein desmosomal complex also includes the cytoplasmic desmosomal plaque proteins plakoglobin, phakophilins, and desmoplakin, which bind to the intracellular domain of the desmogleins and function to anchor the keratin intermediate filament network to site of cellcell contacts.
In human, four desmogleins have been identified (Dsg14). Desmogleins are synthesized with a signal peptide that directs them to the endoplasmic reticulum and a proregion that is removed during protein processing. The mature protein includes four highly conserved extracellular domains (EC 14) and a fifth membrane proximal, more variable EC domain that is referred to as the “extracellular anchor domain. Desmoglein-2 is expressed on various cells including simple epithelia and myocardium, tumors and and many cell cultures.
Desmogleins play critical roles in cell adhesion and skin blistering diseases, as they are the target antigens of autoimmune antibodies and bacterial toxins. Desmosomal dysfunction has been implicated in a number of diseases, including striate palmoplantar keratoderma, skin fragility, and ectodermal dysplasia, and most recently arrhythmic right ventricular cardiomyopathy (ARVC).
Immunogen: Extracellular EC4 domain. Positive Control: A431 Cells. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing DSG2 are readily searchable from our website. Different antibodies against the same target such as DSG2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.