anti-DSG3 antibody product blog
Tags: Antibody; DSG3; Monoclonal Antibody; anti-DSG3 antibody; Desmoglein-3;
The DSG3 dsg3 (Catalog #MBS690868) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human Desmoglein-3 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Desmoglein-3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) - Paraffin. Researchers should empirically determine the suitability of the DSG3 dsg3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The DSG3 dsg3 product has the following accession number(s) (GI #119964718) (NCBI Accession #NP_001935.2) (Uniprot Accession #P32926). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Desmoglein3, also known as pemphigus vulgaris antigen (PVA), is a 130 kDa transmembrane glycoprotein that belongs to the cadherin family of calcium dependent adhesion molecules. Human Desmoglein3 is synthesized with a 26 amino acid (aa) propeptide. The mature protein consists of a 566 aa extracellular domain (ECD) that contains four cadherinlike domains, a 25 aa transmembrane segment, and a 359 aa cytoplasmic domain. Within the ECD, human Desmoglein3 shares 78% aa sequence identity with mouse and rat Desmoglein3. It shares 44%, 47%, and 56% aa sequence identity with Desmoglein1, 2, and 4 respectively. Desmoglein3 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells. It is expressed in the basal and suprabasal layers of stratified epithelia in many tissues. During apoptosis, Desmoglein3 is cleaved by caspases, plus MMP2, and MMP9 at sites within the cytoplasmic and extracellular regions, resulting in shortened transmembrane forms and a soluble 75 kDa ECD fragment. The downregulation of Desmoglein3 in oral squamous cell carcinoma correlates with metastatic potential. Desmoglein3 is the target of autoantibodies in pemphigus vulgaris, a blistering skin disorder with compromised interkeratinocyte adhesion. Binding of these antibodies triggers Desmoglein3 internalization and degradation by keratinocytes.
Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human recombinant Desmoglein-3. Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing DSG3 are readily searchable from our website. Different antibodies against the same target such as DSG3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.