anti-Dynein, Cytoplasmic, 74kD Intermediate Chains antibody product blog
Tags: Antibody; Monoclonal Antibody; Dynein, Cytoplasmic, 74kD Intermediate Chains; anti-Dynein, Cytoplasmic, 74kD Intermediate Chains antibody;
The Dynein, Cytoplasmic, 74kD Intermediate Chains n/a (Catalog #MBS607500) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Dynein, Cytoplasmic, 74kD Intermediate Chains reacts with Bovine, Drosophila, Mouse, Rat, Sheep, Xenopus and may cross-react with other species as described in the data sheet. MyBioSource\'s Dynein, Cytoplasmic, 74kD Intermediate Chains can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC).Suitable for use in Immunocytochemistry, Immunoblot and Immunoprecipitation.
Dilution: Immunocytochemistry: 1:50-1:100. Reacts with cultured MDCK cells, NRR cells, N1E cells and PTK-1 cells.
Immunoprecipitation: 10ul/0.5g tissue. The monoclonal clearly immunoprecipitates the entire dynein complex stoichiometrically from TX-100 or NP-40 lysates (including the 530kD heavy chain, the light intermediate chains and the light chains) from various tissues and cultured cell lines. If detergents such as SDS are used to prepare the lysates the only dynein subunits which are immunoprecipitated are the IC74 subunits (presumably because the dynein complex dissociates), and other contaminating proteins can be found in SDS immunoprecipitates. Researchers should empirically determine the suitability of the Dynein, Cytoplasmic, 74kD Intermediate Chains n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Dynein, Cytoplasmic, 74kD Intermediate Chains with the following immunoassay(s):
Testing Data (1:500 dilution detected DYNEIN on 10ug of A431 lysates. A431 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with DYNEIN (1:500 dilution of this lot). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein DYNEIN (~74kD))
Immunogen: Bovine brain cytoplasmic dynein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Dynein, Cytoplasmic, 74kD Intermediate Chains are readily searchable from our website. Different antibodies against the same target such as Dynein, Cytoplasmic, 74kD Intermediate Chains may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.