anti-EGFR antibody product blog
Tags: Antibody; anti-EGFR antibody; EGFR; Monoclonal Antibody; Epidermal Growth Factor Receptor;
The EGFR egfr (Catalog #MBS632984) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Epidermal Growth Factor Receptor, phosphorylated (Tyr1173) (erbB, EGFR) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Epidermal Growth Factor Receptor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC).Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Dilution: Western Blot: 1:1000
Immunohistochemistry: (Paraffin) 1:25
IHC Protocol: EDTA/TBST
Immunoprecipitation: 1:50. Researchers should empirically determine the suitability of the EGFR egfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The EGFR egfr product has the following accession number(s) (GI #326467049) (Uniprot Accession #P00533). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Epidermal Growth Factor Receptor, phosphorylated (Tyr1173) (erbB, EGFR) with the following immunoassay(s):
Western Blot (WB) (MBS632984 specifically binds to tyrosine phosphorylated EGF receptors but not other phosphorylated tyrosine kinases. Western blot analysis of extracts from cells expressing different activated tyrosine kinase proteins, using MBS632984 (upper) or T9228-75B (lower).)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using MBS632984.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded MDA-MB-468 cells, untreated (left) or EGF-treated (right), using MBS632984.)
Western Blot (WB) (Western blot analysis of extracts from BxPc-3 cells treated with EGF (100 ng/ml for 5 min) or EGF plus the EGF receptor tyrosine kinase inhibitor Gefitinib (10 uM for 2 hrs), using MBS632984 (upper) or EGF Receptor Antibody (lower).)
Epidermal growth factor (EGF) receptor is a 170kD tyrosine kinase. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling and lysosomal degradation (1,2). Phosphorylation of Tyr845 in the kinase domain may stabilize the activation loop, maintain the enzyme in an active state and provide a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of Tyr845 (5). Phospho-tyrosine 992 is a direct binding site for the phospholipase C-g (PLC-g) SH2 domain, resulting in activation of PLC-g-mediated downstreamsignaling (6). Phosphorylation of Tyr1045 creates a major docking site for c-Cbl (7). Binding of c-Cbl to the activated EGF receptor leads to receptor ubiquitination and degradation (8). Phospho-tyrosine 1068 of activated EGF receptor is a direct binding site for Grb2 (9). Phospho-tyrosine 1148 and 1173 provides a docking site for SHC (2). Both sites are involved in activation of MAP kinase signaling. Phosphorylation of EGFR on serine and threonine residues attenuates EGFR kinase activity. Ser1046/1047 in the carboxy-terminal region of EGFR are sites phosphorylated by CaM kinase II. Mutations of Ser1046/1047 upregulate tyrosine autokinase activity of EGFR (10).