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anti-EGFR antibody product blog

Posted on 2016-01-20 18:12:45 by mybiosource_staff
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Tags: Antibody; anti-EGFR antibody; EGFR; Monoclonal Antibody; EGF Receptor;
The EGFR egfr (Catalog #MBS9215527) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EGF Receptor Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s EGF Receptor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP).
WB ~~ 1:1000
ICC ~~ 1:200
IP ~~ 1:500. Researchers should empirically determine the suitability of the EGFR egfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The EGFR egfr product has the following accession number(s) (GI #29725609) (NCBI Accession #NP_005219.2) (Uniprot Accession #P00533). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the EGF Receptor Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot detection of EGFR in A549,MDA-MB-468 and COS7 cell lysates using EGFR mouse mAb(dilution 1:1000).Predicted band size:134 Kda.Observed band size:175KDa.)
Western Blot (WB) EGFR.

Immunocytochemistry (ICC) (Immunocytochemistry staining of HeLa cells using EGFR mouse mAb (dilution 1:200).)
Immunocytochemistry (ICC) EGFR.

Immunoprecipitation (IP) (Immunoprecipitation analysis of Hela cell lysates using EGFR mouse mAb.)
Immunoprecipitation (IP) EGFR.

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.

Antigen Source: Human. Antigen Type: Recombinant Protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing EGFR are readily searchable from our website. Different antibodies against the same target such as EGFR may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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