anti-EGR1 antibody product blog
Tags: Antibody; Monoclonal Antibody; EGR1; anti-egr1 antibody;
The EGR1 egr1 (Catalog #MBS9602645) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EGR1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s EGR1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA).ELISA: 1:10000
WB: 1:500-1:2000
IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the EGR1 egr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The EGR1 egr1 product has the following accession number(s) (GI #4503493) (NCBI Accession #NP_001955.1) (Uniprot Accession #P18146). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the EGR1 Antibody with the following immunoassay(s):
Western Blot (WB) (Figure 1: Western blot analysis using EGR1 mouse mAb against EGR1(AA: 282-433)-hIgGFc transfected HEK293 (1)cell lysate.)
Description: The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene.
Function: Transcriptional regulator (PubMed:20121949). Recognizes and binds to the DNA sequence 5\'-GCG(T/G)GGGCG-3\'(EGR-site) in the promoter region of target genes (By similarity). Binds double-stranded target DNA, irrespective of the cytosine methylation status (PubMed:25258363, PubMed:25999311). Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. Plays a role in the regulation of cell survival, proliferation and cell death. Activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation. Required for normal progress through mitosis and normal proliferation of hepatocytes after partial hepatectomy. Mediates responses to ischemia and hypoxia; regulates the expression of proteins such as IL1B and CXCL2 that are involved in inflammatory processes and development of tissue damage after ischemia. Regulates biosynthesis of luteinizing hormone (LHB) in the pituitary (By similarity).
Subunit Structure: Interacts with SNAI1 and SP1 upon 12-O-tetradecanoylphorbol-13-acetate (TPA) induction.
Similarity: Binds to DNA motifs with the sequence 5\'-GCG(T/G)GGGCG-3\' via its C2H2-type zinc fingers (PubMed:25258363, PubMed:25999311). The first, most N-terminal zinc finger binds to the 3\'-GCG motif, the middle zinc finger interacts with the central TGG motif, and the C-terminal zinc finger binds to the 5\'-GCG motif. Binds double-stranded target DNA, irrespective of the cytosine methylation status. Has reduced affinity for target DNA where the cytosines have been oxidized to 5-hydroxymethylcytosine. Does not bind target DNA where the cytosines have been oxidized to 5-formylcytosine or 5-carboxylcytosine (PubMed:25258363). Belongs to the EGR C2H2-type zinc-finger protein family.