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anti-EIF2A antibody product blog

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Posted on 2017-06-03 08:03:38 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; EIF2A; anti-EIF2A antibody;
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The EIF2A eif2s1 (Catalog #MBS9230947) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EIF2A Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s EIF2A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA).
WB: 1:2000. Researchers should empirically determine the suitability of the EIF2A eif2s1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The EIF2A eif2s1 product has the following accession number(s) (GI #4758256) (NCBI Accession #NP_004085.1) (Uniprot Accession #P05198). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the EIF2A Antibody with the following immunoassay(s):
Western Blot (WB) (All lanes : Anti-EIF2A Antibody at 1:2000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: PC-12 whole cell lysate
Lane 4: NIH/3T3 whole cell lysate

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 36 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB) EIF2A.

Western Blot (WB) (All lanes : Anti-EIF2A Antibody at 1:2000 dilution
Lane 1: K562 whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: PC-12 whole cell lysate
Lane 4: NIH/3T3 whole cell lysate
Lane 5: HepG2 whole cell lysate

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 36 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB) EIF2A.

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Antigen Type: Recombinant Protein
Antigen Source: Human. Cellular Location: Cytoplasmic granule. Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing EIF2A are readily searchable from our website. Different antibodies against the same target such as EIF2A may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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