|The EIF2AK3 eif2ak3 (Catalog #MBS9217062) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EIF2AK3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s EIF2AK3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA).
WB:~~1:250-1:1000. Researchers should empirically determine the suitability of the EIF2AK3 eif2ak3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The EIF2AK3 eif2ak3 product has the following accession number(s) (GI #134304838) (NCBI Accession #NP_004827.4) (Uniprot Accession #Q9NZJ5). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the EIF2AK3 Antibody with the following immunoassay(s):
Western Blot (WB) (All lanes : Anti-EIF2AK3 Antibody at 1:250-1:1000 dilution
Lane 1: A549 whole cell lysate
Lane 2: PC-12 whole cell lysate
Lane 3: mouse brain lysate
Lane 4: mouse liver lysate
Lysates/proteins at 20 ug per lane.
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 125 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.)
Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1(CCND1). Involved in control of mitochondrial morphology and function (By similarity).
Cellular Location: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Function: Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2Sl) on \'Ser-52\' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2Sl either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced Gl growth arrest due to the loss of cyclin-Dl (CCNDl). Involved in control of mitochondrial morphology and function.
Tissue Location: Ubiquitous. A high level expression is seen in secretory tissues. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing EIF2AK3 are readily searchable from our website. Different antibodies against the same target such as EIF2AK3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.