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anti-ENO1 antibody product blog

Posted on 2015-09-19 00:44:36 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; ENO1; anti-ENO1 antibody;
The ENO1 eno1 (Catalog #MBS415380) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ENO1 Antibody reacts with Human, bovine, mouse, rat and Xenopus sources and may cross-react with other species as described in the data sheet. MyBioSource\'s ENO1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunocytochemistry (ICC).
Try at dilutions of 1:500-1:1,000 for immunofluorescence. For western blots try at 1:5,000. The NNE protein runs at about ~47 kDa on SDS-PAGE gels. Researchers should empirically determine the suitability of the ENO1 eno1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The ENO1 eno1 product has the following accession number(s) (GI #39644850) (NCBI Accession #AAH09218.2) (Uniprot Accession #P06733). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ENO1 Antibody with the following immunoassay(s):
Western Blot (WB) #2
Western Blot (WB) #2 ENO1.

Testing Data
Testing Data ENO1.

Antibody Type: Primary antibody
Immunogen: Enolases are enzymes which catalyze the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. The Enzyme Commission classification number for this class of enzyme is EC4.2.1.11, and they can also be refered to as 2-phospho-D-glycerate hydrolases. There are three enolase proteins each coded for by a distinct gene. They are known as enolase 1, 2 and 3 or alternately as α, beta and gamma-enolase. Confusingly, although enolase 1 is alphaenolase, enolase 2 corresponds to gamma-enolase while enolase 3 is also known as beta-enolase. All three enzymes exist as dimers in vivo and the three molecules are very similar in primary amino acids sequence, as can be seen from a sequence alignment downloadable from here. This similarity allows not only homodimers but also most possible heterodimers to form. The expression pattern of the three enzymes differs in a tissue specific manner, so that antibodies to them are useful cell type specific markers, especially as all three molecules are very abundant. Enolase 1 or α is also known as non-neuronal enolase (NNE) and is expressed in most kinds of tissue, but is absent from neurons. Abnormal expression of NNE is associated with tumor progression in some breast and head and neck cancer (1, 2). Enolase 2 or gamma is also known as neuron specific enolase (NSE), and we also market antibodies directed against this protein, specifically RPCA-NSE. A switch from NNE to NSE occurs in the development of neurons (3). Enolase 3 or beta is expressed primarily in muscle cells. Monoclonal antibody MCA-253 was raised against the N-terminal 12 amino acids of bovine enolase 1 which was synthesized on a 8-amine lysine core using the multiple antigen presentation method (5). This produces a dendrimer presenting 8 peptides to the immune system. The characterization of the antibody has been published in peer reviewed form and in this publication is there referred to as Enol2-53 (4). The antibody was tested for binding to expressed bovine enolase 1, 2 and 3 and shown to be specific for only enolase 1. The HGNC name for this protein is ENO1.
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