anti-EOMES antibody product blog
Tags: Antibody; Monoclonal Antibody; EOMES; anti-EOMES antibody;
The EOMES eomes (Catalog #MBS6010440) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EOMES (Eomesodermin Homolog, T-box Brain Protein 2, T-brain-2, TBR-2, TBR2) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s EOMES can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS).Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunocytochemistry.
Dilution: Western Blot: 1ug/ml
Immunocytochemistry: 8-25ug/ml
Flow Cytometry: 2.5ug/106 cells. Researchers should empirically determine the suitability of the EOMES eomes for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The EOMES eomes product has the following accession number(s) (GI #22538470) (NCBI Accession #NP_005433.2) (Uniprot Accession #O95936). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the EOMES (Eomesodermin Homolog, T-box Brain Protein 2, T-brain-2, TBR-2, TBR2) with the following immunoassay(s):
Testing Data (EOMES in Mesodermally Differentiated BG01V Human Stem Cells. EOMES was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using MBS6010440 at 10ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLightsTM 557conjugated Mouse IgG Secondary Antibody and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.)
EOMES is a 72kD member of the TBR1 subfamily, T-box family of transcription factors. It is expressed in NK and CD8+ T cells, where CTLA4 activation suppresses EOMES activation of IFN-g and granzyme B genes. It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation. Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm. Here, it synchronizes a multicellular commitment to a cell lineage.
Immunogen: Recombinant corresponding to aa471-686 from human EOMES expressed in E. coli (O95936). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing EOMES are readily searchable from our website. Different antibodies against the same target such as EOMES may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.