anti-EPCAM antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-Epcam antibody; Epcam;
The EPCAM epcam (Catalog #MBS9602424) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EPCAM Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s EPCAM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA).ELISA: 1:10000
WB: 1:500-1:2000
IHC: 1:200-1:1000. Researchers should empirically determine the suitability of the EPCAM epcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The EPCAM epcam product has the following accession number(s) (GI #218505670) (NCBI Accession #NP_002345.2) (Uniprot Accession #P16422). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the EPCAM Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis using EPCAM mouse mAb against A431 (1), MCF-7 (2) cell lysate.)
Description: This 9-exon gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas.
Function: May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins An and E.
Subunit Structure: Monomer. Interacts with phosphorylated CLDN7.
Post-translational Modifications: Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.
Similarity: Belongs to the EPCAM family.
Immunogen: Purified recombinant fragment of human EPCAM expressed in E. Coli
Subcellular Location: Lateral Cell Membrane. Cell Junction > Tight Junction. Co-localizes with CLDN7 at the lateral cell membrane and tight junction.
Tissue Specificity: Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC\'s differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing EPCAM are readily searchable from our website. Different antibodies against the same target such as EPCAM may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.