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anti-EphA3 antibody product blog

Posted on 2015-07-21 10:00:50 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; EphA3; anti-EphA3 antibody;
The EphA3 epha4 (Catalog #MBS850771) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EphA3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s EphA3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA).
WB: 1/500 - 1/2000, ELISA: Propose dilution 1/10000
Not yet tested in other applications. Determining optimal working dilutions by titration test. Researchers should empirically determine the suitability of the EphA3 epha4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The EphA3 epha4 product has the following accession number(s) (GI #4758280) (NCBI Accession #NP_004429.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the EphA3 Antibody with the following immunoassay(s):
Testing Data (Figure 1. Western blot analysis using EphA3 mouse mAb against truncated Trx-EphA3 recombinant protein (1) and truncated EphA3(aa566-983)-hIgGFc transfected CHO-K1 cell lysate(2).)
Testing Data EphA3.

Background: EphA3. EPH receptor A3. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-An and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene.

Immunogen: Purified recombinant fragment of EphA3 (aa751-983) expressed in E. Coli.
Species: Mouse IgG2b. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing EphA3 are readily searchable from our website. Different antibodies against the same target such as EphA3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Brain, Embryonic Tissue, Eye, Heart, Kidney, Muscle, Skin, Vascular tissues are correlated with this protein. EphA3 also interacts with the following gene(s): EFNA1, EFNA2, EFNA3, EFNA4, EFNA5, EFNB2, FYN, NGEF, SRC. Adenocarcinoma, Disease Models, Animal, Embryo Loss, Fetal Death, Immune System Diseases, Inflammation, Necrosis, Nervous System Diseases, Prostatic Diseases, Prostatic Neoplasms are some of the diseases may be linked to EphA3 Antibody.
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