anti-EPOR antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-EPOR antibody; EPOR; CLAUDIN6/9;
The EPOR epor (Catalog #MBS468036) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The anti-human Claudin 6 and Claudin 9 monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CLAUDIN6/9 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), CELISA.ELISA: 1:200 - 1:400
Flow Cytometry: 1.2 ug/10^6 cells
CELISA: 1:200 - 1:400. Researchers should empirically determine the suitability of the EPOR epor for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The EPOR epor product has the following accession number(s) (GI #4503591) (NCBI Accession #NP_000112.1) (Uniprot Accession #P19235). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human Claudin 6 and Claudin 9 monoclonal antibody with the following immunoassay(s):
Testing Data (Fig.1: GM-1111. BOSC23 cells were transiently transfected with an expression vector encoding either Claudin 9 (red curve), Claudin 6 (green curve) or an irrelevant protein (control transfectant). Binding of YD-9H8 was detected with a PE conjugated secondary antibody. A positive signal was obtained with Claudin 9 and Claudin 6 transfected cells.)
Testing Data #2 (Fig.2: SDS-PAGE analysis of purified YD-9H8 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified YD-9H8 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Background: Claudin 6 and Claudin 9 belong to the claudin family which constitutes a large group of fourtransmembrane domain proteins (1). Claudins are integral tight junction proteins that are responsible for maintaining the integrity of epithelial cell architecture, the control of paracellular transport and cell polarity. The expression pattern of claudins is tissue specific, most tissues express multiple claudins, which can interact in both homotypic and heterotypic fashion to form the tight junction strands (2). Several claudin proteins have been shown to be abnormally expressed in cancers. The differential expression of these proteins between tumour and normal cells, in addition to their membrane localisation, makes them prime candidates for cancer therapy (2). Members of the claudin family, including Claudin 6 and 9 are expressed in the liver and play a critical role in Hepatitis C Infection. They function as coreceptors in HCV entry and assume a role in HCV dissemination, replication and pathogenesis (3).
Buffer: phosphate buffered saline, pH 7.2
Immunogen: genetic immunisation with cDNA encoding human Claudin 9
Selection: based on recognition of the complete native protein expressed on transfected mammalian cells
Immunogen: cDNA. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing EPOR are readily searchable from our website. Different antibodies against the same target such as EPOR may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.